Kate Tolo's Biohacking Protocol Runs on $2.6 Million From Immortals

Kate Tolo is tracking more than 1,500 biomarkers under a $2.6 million research budget from Immortals, the wellness company she co-founded with her boyfriend Bryan Johnson, according to Wired. The 30-year-old has adopted a self-tracking protocol modeled on Johnson's own, which he says is meant to serve "half the population."

How much funding backs the protocol?

Immortals, the company Tolo and Johnson founded together, has put $2.6 million behind her testing regimen, Wired reported. The money funds a battery of blood, urine, and stool panels drawn from what Wired describes as "an elaborately choreographed four-hour techno ballet of scratching, sniffing, spitting, scanning, stretching, gripping, vibrating, flexing, plucking, poking, pulling, peeing, and pooping," a description the outlet attributes to a Business Insider profile of the routine. Johnson made his own fortune before turning to public self-optimization; Immortals is the vehicle through which the couple is now applying that model to Tolo, a former fashion-marketing employee who joined Johnson's neurotech company, Kernel, as his executive assistant and special-projects director in 2021.

What does Tolo track each cycle?

The protocol pulls more than 1,500 biomarkers across the course of Tolo's menstrual cycle, per Wired's reporting. That includes tracking nighttime erections — a metric more commonly associated with male physiology — and subjecting herself to extreme heat while menstruating, part of an effort to build a data set on a cycle Wired notes is far less studied in biohacking circles than the markers Johnson tracks on himself. Johnson has called her "the world's most measured woman," a label he used in a video posted last August, according to Wired.

What is endometriosis, and why does it matter here?

Endometriosis is a chronic disease caused by an overgrowth of endometrium-like tissue outside the uterus, and it affects an estimated 10 percent of women of reproductive age, according to World Health Organization data cited by Wired. Symptoms include heavy and painful periods, pelvic floor dysfunction, bowel issues, and pain during sex. Tolo said a doctor suspected she had the condition in her early twenties after she was rushed to the emergency room with severe pain, and recommended a laparoscopy — an invasive surgical procedure required for an official diagnosis and used to remove endometriosis tissue. She declined. "I wasn't going to undergo an invasive surgical procedure, or cut myself open," Tolo told Wired. "That just wasn't an option for me." She said she raised the history during a clinical intake for the couple's concierge health service in early 2026, and Johnson became fixated on finding a cure.

How does Johnson describe her role?

Johnson has framed Tolo's participation as data collection on behalf of women broadly, not just her own health optimization. He has also made specific claims about her results, telling followers he considers her vaginal microbiome to be in the "top 1 percent" of all vaginal ecosystems, according to Wired.

"He's also declared her vagina's microbiome to be in the 'top 1 percent' of all vaginal ecosystems."

Wired did not report an independent source verifying that ranking or the methodology behind it.

What did Tolo say about the physical and mental toll?

Tolo told Wired she is prone to depression and anxiety and had a panic attack in the second week of the protocol, triggered when her internet connection dropped during a red-light therapy session. "It's a deeply uncomfortable experience for someone who doesn't love a routine," she said. "And so all of that pressure just really got to me." She described herself as an introvert who was initially uneasy about becoming the subject of such intensive measurement, though she said the process has ultimately felt worthwhile given her own history with undiagnosed pain dating back to her first period at age 13.

What to watch

• Whether Immortals publishes any of the 1,500-plus biomarker data points or methodology publicly, rather than through social-media claims.
• Whether Tolo eventually pursues the laparoscopy she has so far declined, or whether the protocol produces a non-surgical diagnostic path.
• Whether Johnson's endometriosis research effort results in any peer-reviewed findings, given the WHO's 10 percent prevalence estimate cited in the Wired report.
• Whether other women's-health biohacking ventures cite Tolo's protocol as a template, or criticize its lack of independent verification.

Wired's full profile, including additional detail on the testing schedule and the couple's relationship timeline, is available at the originating story.